The Effects of Exercise on Spleen Fibrosis and Macrophage Number in D-Galactose-Induced Aging Rat Model

Background: Spleen plays a role in human immune system as well recycling and filtering blood. The aging spleen is associated with fibrosis impaired system, increasing individual vulnerability to getting infections. Regular physical activity essential maintain enhance body fitness, endurance, system.Objective: This study aimed investigate the effects of mild moderate-intensity treadmill exercise on by examining number macrophages d-Galactose-induced rat models.Methods: Twenty-four male Wistar rats were randomly divided into four groups: G1 (control group), G2 (d‐Galactose, no exercise), G3 low-intensity G4 exercise). d-Galactose was administered intraperitoneally day 0 given for weeks following modified Brown et al. (2007) protocol. Spleens histologically processed stained picrosirius red against CD68+ antibody. Percentage fraction area macrophage cell count obtained using ImageJ, data analyzed statistically SPSS software.Results: did not show any differences weight, length, width among groups (P > 0.05). administration causes an increased macrophages. Low could lower percentages area. However, effective lowering number.Conclusion: Moderate-intensity induced d-Galactose.